Sensitive and specific detection of Xanthomonas axonopodis pv. citri by PCR using pathovar specific primers based on hrpW gene sequences
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چکیده
منابع مشابه
Transformation of Xanthomonas axonopodis pv. citri by Electroporation
This study describes the use of electroporation for transforming Xanthomonas axonopodis pv. citri (Xac), the causal agent of citrus (Citrus spp.) canker. It also evaluates the methodology used for this species under different electrical parameters. The bacterium used in the study (Xac 306) was the same strain used for recent complete sequencing of the organism. The use of a plasmid (pUFR047, ge...
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Strains of the plant-pathogenic bacterium Xanthomonas axonopodis pv. citri are differentiated into two groups with respect to aggressiveness (normal and weak) on Citrus grandis cultivars but not on other Citrus species such as Citrus sinensis. Random mutagenesis using the transposon Tn5 in X. axonopodis pv. citri strain KC21, which showed weak aggressiveness on a C. grandis cultivar, was used t...
متن کاملIdentification and characterization of a second lexA gene of Xanthomonas axonopodis Pathovar citri.
We previously identified and characterized a lexA gene from Xanthomonas axonopodis pv. citri. For this study, we cloned and expressed a lexA homologue from X. axonopodis pv. citri. This gene was designated lexA2, and the previously identified lexA gene was renamed lexA1. The coding region of lexA2 is 606 bp long and shares 59% nucleotide sequence identity with lexA1. Analyses of the deduced ami...
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Xanthomonas hortorum pv. pelargonii is the causal agent of bacterial blight of geranium. A specific and rapid real-time PCR assay for detecting the bacterium in plant was developed in this study. We compared sensitivity of conventional and real-time PCR for detection of the pathogen in inoculated plants. For application to disease management programs, PCR amplification must be able to detect la...
متن کاملPrimers based on the rpf gene region provide improved detection of Xanthomonas axonopodis pv. citri in naturally and artificially infected citrus plants.
AIMS To have a PCR-based detection method for Xanthomonas axonopodis pv. citri (Xac) using primers designed in a specific region of its genome. METHODS AND RESULTS A Xac-specific region was identified inside the rpf gene cluster of strain IAPAR 306 in an analysis of its complete genomic sequence. Two primers were designed, Xac01 and Xac02, which, when used in a standard PCR assay, direct the ...
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ژورنال
عنوان ژورنال: Microbiological Research
سال: 2006
ISSN: 0944-5013
DOI: 10.1016/j.micres.2005.07.005